Technology/ Title:Compositions and Methods of Detection of Protein S-nitrosylation and Oxidation : PAC-switch method
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Technology Description
Abstract：
The primary focus of this invention is to develop a new analytic platform, which is technique friendly and at the same time greatly enhances the specificity and accuracy of S-nitrosylated proteins being identified. In this invention, we established an antibody-based strategy. Sequential blocking steps successfully eliminate false-positive results. This invention also provides the application of detection in both biochemical and cell imaging formats.
Fields of Application：
1. Detection of nitrosylation and oxidation of proteins.
2. Screening of potential drugs useful for modulating protein nitrosylation and oxidation.
Advantages when compared to the existing technologies：
high specificity and accuracy (reduce the false-positive results)
Intellectual Property
Patents:
TW I585107
US 10072097 B2
EU Pending
Key Publications:
Hsu MF, Pan KT, Chang FY, Khoo KH, Urlaub H, Cheng CF, Chang GD*, Haj FG*, and Meng TC S-Nitrosylation of endogenous protein tyrosine phosphatases in endothelial insulin signaling. Free Rad Biol Med (2016)
Business Opportunity
Technical transfer or cooperative research /development.
